Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction).
Nagler Medium
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Product Description
| Organisms | Ref. No | Result |
| Clostridium perfringens | NCTC 8237 | Growth with opalescent zone (+ve reaction) |
| Escherichia coli | NCTC 12241 | Growth with no zone (-ve reaction) |
