Fastidious Anaerobe Agar

Fastidious Anaerobe Agar

KM0006 Fastidious Anaerobe Agar is a general-purpose medium, when supplemented with blood and any necessary selective agents, can be used for the isolation and culture of fastidious anaerobes from clinical specimens.

Anaerobic infections are common infections caused by anaerobic bacteria. These bacteria occur naturally and are the most common flora in the body; in their natural state, they do not cause infection. Anaerobic bacteria can infect deep wounds, tissue, and internal organs where there is little oxygen after injury or surgery. Infection by anaerobic bacteria is normally characterized by formation of abscesses, foul smelling discharge, pus, and free gas in tissue, leading to tissue degradation.

KM0006 is recommended as a standard, primary isolation or supplementary medium by the UK Standards for Microbiology Investigations and tested in accordance with the principles of ISO 11133:2014.

Related Supplements :
LS0015 Actinomycete Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficile Selective Supplement, LS0023 Clostridium perfringens Selective Supplement, BM0140 Egg Yolk Emulsion

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

Prepared with 7% Defibrinated Horse Blood

Organisms Ref. No Result
Clostridium perfringens NCTC 8237 Growth: Grey colonies
Clostridium difficile NCTC 11204 Growth: Grey colonies
Bacteriodes fragilis NCTC 9343 Growth: Grey colonies
Fusobacterium nucleatum NCTC 10562 Growth: Pale yellow colonies
Actinomyces israelii NCTC 10236 Growth: Pale yellow colonies
Peptostreptococcus anaerobius ATCC 27337 Growth: Grey colonies

 

Preparation

Suspend 46g/l of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add the appropriate volume of sterile defibrinated horse or sheep blood. Various selective supplements, such as neomycin (LS0017), may also be aseptically added at this point to create a variety of selective blood agar products. Gently homogenize the final medium and dispense into appropriate sterile containers.

Formula

Ingredient *  g/L
Peptone 23.0
Soluble starch 1.0
Sodium chloride 5.0
Sodium bicarbonate 0.4
Glucose 1.0
Sodium pyruvate 1.0
L-Cysteine HCl 0.5
L-Arginine 1.0
Haemin 0.01
Vitamin K 0.001
Soluble pyrophosphate 0.25
Sodium succinate 0.5
Agar 12.0
Total 46.0
* Adjusted / supplemented as required to meet performance requirements