Malt extract broth is used for the cultivation of yeasts and moulds and is commonly used as part of sterility testing protocols. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds.
Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth.
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
| Organisms | Ref. No | Result |
| Candida albicans | NCPF 3179 | Turbid growth |
| Aspergillus brasiliensis | NCPF 2275 | Surface growth |
| g/L | |
| Malt extract | 17.0 |
| Mycological peptone | 3.0 |
| Total | 20.0 |
| *Adjusted/supplemented as required to meet performance requirements | |
Suspend 20g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 115°C for 10 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into final containers. Protracted boiling or prolonged holding at elevated temperature induces precipitation.