Mueller Hinton Agar

SKU: KM0183 Category: Dehydrated Culture Media Tag: aquaculture

Mueller Hinton agar is used for antimicrobial susceptibility testing by the disk diffusion method as described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST).
This formulation conforms to Clinical and Laboratory Standard Institute (CLSI). Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium. Starch absorbs any toxic metabolites produced. The medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions.
The base medium can be supplemented with blood (5 10%). Nicotinamide adenine dinucleotide (NAD) may be added at 20 mg/L to make the medium suitable for use with more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae (LS0005).

Related Supplements :
LS0005 NAD Supplement (20mgs/L), Defibrinated Horse Blood

Additional Information

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Straw
Prepared Medium Colour	Plain medium: straw. With Blood: Red.
Dehydrated PH	7.3 ± 0.1
Prepared PH	7.3 ± 0.1
Recommended Incubation	Plain medium: aerobically at 37°C ± 1°C for 16-20 hours. Blood medium: Recommended incubaiton will depend on the methodology being used.

Product Description

Organisms	Ref. No	Result
Staphylococcus aureus (no supplementation)	ATCC 25923	Growth, white colonies
Escherichia coli (no supplementation)	ATCC 25922	Growth, grey colonies
Pseudomonas aeruginosa (no supplementation)	ATCC 27853	Growth, cream colonies
Neisseria gonorrhoeae (with 5% horse blood & NAD)	NCTC 8375	Growth, grey/opaque colonies
Haemophilus influenzae (with 5% horse blood & NAD)	NCTC 8468	Growth, grey/opaque colonies
Streptococcus pneumoniae (with 5% horse blood & NAD)	NCTC 12977	Growth, green colonies with α-haemolysis

Formula*

	g/L
Beef extract	2.0
Acid hydrolysate of casein	17.5
Starch	1.5
Agar	17.0
Total	38.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 38g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C. If preparing a blood Mueller Hinton plate after cooling aseptically add 50 ml/l of defibrinated horse blood and 2 vials of LS0005. Allow to mix and aseptically dispense into appropriate sterile containers.

Mueller Hinton Agar