Listeria Oxford Selective Supplement
E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| pH | |
| Solubility Description | |
| Recommended Incubation | Incubate plates at 35-37°C in aerobic atmosphere for 48 hours |
| Organisms | Ref. No | Result |
| Tested as PP0630 (Listeris Oxford Agar) | ||
| Listeria monocytogenes | NCTC 11994 | Growth & Black colonies |
| Escherichia coli | NCTC 12241 | Inhibited |
| Enterococcus faecalis | NCTC 12697 | Inhibited |
| Candida albicans | NCPF 3179 | Inhibited |
To reconstitute the contents of each vial, aseptically add 3ml of Sterile Deionised Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be an opaque, yellow colour and free from visible particulate matter. Immediately add the contents of the vial to 500ml of sterile Listeria Oxford agar prepared according to the manufacturer’s instructions and allowed to cool to 45- 50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes. Overnight storage at 2 – 8°C is recommended to allow equilibration of the supplement within the medium.
| Vial contents: | |
| Colistin sulphate | 10.0mg |
| Amphotericin B | 5.0mg |
| Cefotetan | 1.0mg |
| Phosphomycin | 5.0mg |
| Acriflavine | 2.5mg |