Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
This balanced salt solution is issued to meet the requirements of those tissue culture workers who use the Dulbecco Solution with and without calcium and magnesium.
Phosphate Buffered Saline (PBS)
Additional Information
| Container | |
|---|---|
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH |
Product Description
| Organisms | Ref. No | Result |
| Escherichia coli | NCTC 12241 | Stable viability* |
| Staphylococcus aureus | NCTC 12981 | Stable viability* |
| *Stable viability = ≤ ± 30% difference compared to original count after incubation for 45 minutes at 20-25°C |
Formula*
| g/L | |
| Potassium chloride | 0.2 |
| di Sodium Hydrogen phosphate anhydrous | 1.15 |
| Sodium chloride | 8.0 |
| Potassium di hydrogen phosphate | 0.2 |
| Total | 9.55 |
| *Adjusted/supplemented as required to meet performance requirements | |
Preparation
Suspend 9.55g of the medium in one litre of deionised / purified water. Allow the medium to dissolve whilst mixing for 10 minutes. Dispense the required volume into the final containers and sterilise at 121°C for 15 minutes.