This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli.
It should be noted that AmpC isolates may also be detected on this medium whilst non – ESBL organisms will be inhibited on this medium.
The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow.
N.B. – This is double strength broth.
MacConkey Broth (Double Strength) with Cefotaxime (2mg/L)
Additional Information
| Container | |
|---|---|
| Volume | |
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Appearance | Liquid |
| Colour | |
| pH | |
| Recommended Incubation | Incubate aerobically at 37 ± 1 oC or 44 ± 1 oC (depending on methodology) for 24-48 hours. |
Product Description
| Organisms | Ref. No | Result |
| Escherichia coli (ESBL) | NCTC 13441 | Growth (Yellow colour in broth) |
| Proteus mirabilis | NCTC 10975 | Inhibited |
| Escherichia coli | NCTC 12241 | Inhibited |
| Staphylococcus aureus | NCTC 12981 | Inhibited |
Recommended incubation: Incubate aerobically at 37 ± 1oC or 44 ± 1oC (depending on methodology) for 24-48 hours.
