BM1436 Tryptone Soya Agar (pH 7.2) is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. This medium meets the requirements of The British Standards Institution (1) and is tested according to the principles of ISO 11133:2014 (2). and is based on the original formulation described by Leavitt et al. in 1955 (3). In environmental monitoring applications, it is common for plates to be incubated at 30-35°C for bacterial colonies and 20-25°C for mould and fungi (4).
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
- International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media. Geneva, ISO.
- Leavitt, J., Naidorf, I. and Shugaevsky, P. (1955) Aerobes and anaerobes in endodontics. Part I. The undetected anaerobes in endodontics. Part II. Sensitive culture medium for the detection of both aerobes and anaerobes. NYSDJ, 25, pp.377-382.
4. Anon. (1987) Testing methods for use in quality assurance of culture media. Int. J. Food Microbiol., 5, pp.291-296
