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Soy peptone is manufactured from the enzymatic hydrolysis of soybean. This product provides a good source of nitrogen, carbohydrates, and vitamins. It is recommended for use in microbiological media for the detection and isolation of a wide variety of bacteria and fungi. -
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical and food samples. The formulation was developed by Kobayashi et al. which was modified from Nakanishi’s formulation. Vibrio species are most widely recognized for their role in human intestinal infections and cholera worldwide. Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is recommended by the FDA BAM, the UK Standards for Microbiology Investigations, and complies with the standard laid out by ISO 21872.
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Trichomonas medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. (1) The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of trichmonads. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The addition of selective agents such as chloramphenicol is recommended to inhibit bacterial species that may be present in specimens. References 1) Kupferberg, A.B. Johnson, G., and Sprince, H. 1948. Proc. Soc. Exper. Biol. Med., 67:304-308
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Triple sugar iron agar is used to differentiate between some of the enterobacteriacae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide. Beef extract, yeast extract and peptone provide the required nitrogen, carbon and vitamins. Lactose, sucrose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains osmotic balance.
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Tryptone is obtained by pancreatic digestion of casein. Casein is the main protein of milk and is a rich source of amino acid and nitrogen. This product can be used in preparing microbiological culture media providing nitrogen, vitamins, minerals and amino acids. Due to the high tryptophan content in tryptone it can be used in detecting indole production.
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Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli without the need for membranes or pre-incubation. Based on the formulation of Tryptone Bile Agar it incorporates a chromogenic substrate, X-Glucuronide, to detect the ß-glucuronidase enzyme which is specific for the majority of E. coli strains. Approximately, 3-4% of E. coli are glucuronidase negative including E. coli O157.(1) The advantage of the chromogenic substrate is that the reaction is concentrated within the colony resulting in distinctive blue/green colonies of E. coli while the other coliforms produce cream colonies. The tryptone provides the required carbon, nitrogen and vitamins. Bile salts No.3 is a selective agent against Gram-positive bacteria. X-glucuronide is a chromogenic substrate. Agar is solidifying agent.
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Tryptone Soya Agar is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or simply general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. Tryptone Soya Agar may be used to determine X and V factor requirements of Haemophilus species; sterility testing; and environmental monitoring within pharmaceutical cleanrooms and sterile facilities. This medium meets the requirements of the Harmonized USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. Tryptone Soya Agar is recommended as a reference medium when testing selective media, to measure the degree of inhibition. In environmental monitoring applications, it is common for plates to be incubated at 30-35°C for bacterial colonies and 20-25°C for mould and fungi. Tryptone Soya Agar will support the growth of both aerobic and anaerobic organisms depending on incubation conditions. KM0024 unsupplemented is recommended by the World Health Organization, UK Standards for Microbiology Investigations, International Organization for Standardization and is tested in accordance with ISO 11133:2014. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing. UK Standards for Microbiology Investigations also call for Tryptone Soya Agar supplemented with 5% sheep blood (E&O DSC) for aid in the identification of Bordetella species from clinical specimens. The addition of defibrinated animal blood to the base medium, promotes the growth of most fastidious organisms and presumptive identification can be made based on haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood. Addition of selective agents allows isolation and presumptive identification of specific species or groups of organisms. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood
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A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
