Alkaline Peptone Water (pH 8.6)

Alkaline Peptone Water (pH 8.6)

Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces.
The high pH of the medium inhibits most enteric organisms for at least 24 hours.
The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar.
This enrichment medium is also used for food and water testing.

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Product Description

Organisms Ref. No Result
Vibrio furnissii NCTC 11218 Growth & turbidity
Vibrio parahaemolyticus NCTC 10885 Growth & turbidity

 

Recommended incubation: Incubate at 37 ± 1°C for 6 hours.
Subculture 10μl of the productivity broths onto TCBS agar and incubate at 37 ± 1°C for 18-24 hours.