Trichomonas Broth (CPLM) with Nystatin

This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of two selective agents namely, Chloramphenicol and Nystatin, to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.