This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation.
Tryptone Soya Agar with Lecithin (0.7g/L) & Tween 80 (5.0g/L)
Additional Information
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Recommended Incubation | A. brasiliensis – aerobically at 20-25°C for 72-120 hours, B. subtilis, Ps. Aeruginosa, E. coli and S. aureus, Aerobically at 30-35°C for 18-24 hours, C. albicans – aerobically at 20-25°C for 48 hours |
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Product Description
Organisms | Ref. No | Result |
Staphylococcus aureus | ATCC 6538 | Yellow colonies |
Escherichia coli | ATCC 8739 | Cream colonies |
Pseudomonas aeruginosa | ATCC 9027 | Cream colonies |
Aspergillus brasiliensis | ATCC 16404 | White colonies, reverse side is pale yellow |
Candida albicans | ATCC 10231 | White colonies |
Bacillus subtilis | ATCC 6633 | White colonies |