Sabouraud Dextrose Agar

KM0003 Sabouraud Dextrose Agar powder is a general-purpose, non-selective medium which is used for the isolation of yeasts and moulds from clinical, food, pharmaceutical and cosmetic samples.

Sabouraud Dextrose Agar is a modification of a medium originally described by Sabouraud. The fungi maintain their typical cultural appearance and thus may be readily identified according to the standard macroscopic characters described by Sabouraud. The formulation conforms to European, United States and Japanese Pharmacopeia requirements. This medium complies with ISO 11133:2014, where it is described as the main reference medium to carry out quantitative testing on culture media intended for fungi.

Sabouraud Dextrose Agar is recommended by the UK Standards for Microbiology Investigations for various purposes, including as a standard, supplementary or optional medium.

Related Supplements :
LS0050 Chloramphenicol Selective Supplement

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

Organisms Ref. No Result
Trichophyton rubrum NCPF 419 Growth: White fluffy colonies with dark red/brown underside (ISO)
Aspergillus brasiliensis NCPF 2275 Growth: White colonies with black pigmentation, pale yellow reverse (USP, ISO)
Candida albicans NCPF 3179 Growth: White colonies (USP, ISO)
Aspergillus fumigatus DSMZ 819 Growth: White colonies with blue/green pigmentation, pale yellow reverse (ISO)
Saccharomyces cerevisiae NCPF 3178 Growth: White colonies (ISO)

 

Formula*

Formula * g/L
Tryptone 5.0
Meat peptone 5.0
Dextrose 40.0
Agar 15.0
Total 65.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 65g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.
NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.