Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical and food samples. The formulation was developed by Kobayashi et al. which was modified from Nakanishi’s formulation. Vibrio species are most widely recognized for their role in human intestinal infections and cholera worldwide. Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is recommended by the FDA BAM, the UK Standards for Microbiology Investigations, and complies with the standard laid out by ISO 21872.
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated pH | |
| Prepared pH | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Vibrio parahaemolyticus | NCTC 10885 | Growth: Green colonies |
| Vibrio furnissii | NCTC 12218 | Growth: Yellow colonies |
| Vibrio cholerae | NCTC 11348 | Growth: Yellow colonies |
| Escherichia coli | NCTC 12241 | Inhibited |
| Proteus mirabilis | NCTC 10975 | Inhibited |
| Pseudomonas aeruginosa | NCTC 12903 | Inhibited |
| Aeromonas hydrophila | NCTC 8049 | Inhibited |
Formula*
| g/L | |
| Yeast extract | 5.0 |
| Peptone | 10.0 |
| Sodium thiosulphate | 10.0 |
| Sodium citrate | 10.0 |
| Ox bile | 8.0 |
| Sucrose | 20.0 |
| Sodium chloride | 10.0 |
| Ferric citrate | 1.0 |
| Bromothymol blue | 0.04 |
| Thymol blue | 0.04 |
| Agar | 11.0 |
| Total | 85.0 |
| *Adjusted/supplemented as required to meet performance requirements |
Preparation
Suspend 85g/l of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation until boiling. Cool to 45-50°C and aseptically dispense into appropriate sterile containers
NB: Protracted boiling or prolonged holding at elevated temperature induces precipitation.
