Helicobacter Pylori Agar

This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with charcoal, ferrous sulphate and sodium pyruvate replacing the horse blood. The medium is made selective by the addition of vancomycin and cefsulodin, to suppress other bacteria, and amphoteracin to inhibit yeasts. Horse serum (10%) is also added to promote optimum growth of Helicobacter spp.

Additional Information

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Black
Prepared Medium Colour	Black
Dehydrated PH	7.4 ± 0.2
Prepared PH	7.4 ± 0.2
Recommended Incubation	Aerobically at 35-37°C for 48-72 hours

Product Description

Organisms	Ref. No	Result
Helicobacter pylori	NCTC 11916	Growth, grey colonies
Enterococcus faecalis	NCTC 12697	Inhibited
Candida albicans	NCPF 3179	Inhibited

Formula*

	g/L
Peptone	23.0
Sodium chloride	5.0
Charcoal	4.0
Ferrous sulphate	0.25
Sodium pyruvate	0.25
Agar	12.0
Sodium carbonate	0.5
Total	45.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 45 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. and add two vials of Helicobacter pylori selective supplement (LS0031) and 10% horse serum, mix well and aseptically dispense into appropriate sterile containers.

Helicobacter Pylori Agar