MacConkey Agar without Salt and Crystal Violet

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MacConkey Agar without Salt and Crystal Violet

MacConkey Agar without Salt and Crystal Violet, based on the formulation by Rappaport and Henig, is a differential medium for the isolation and differentiation of lactose and non-lactose fermenting enteric bacteria while also restricting the swarming of Proteus species. KM0011 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations, The Microbiology of Drinking Water and tested in accordance with ISO 11133:2014.

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LS0189 Cefotaxime Supplement (1mg/L)

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth: Pink colonies
Escherichia coli NCTC 12241 Growth: Red colonies
Proteus mirabilis NCTC 10975 Growth: Yellow colonies
Proteus mirabilis NCTC 11938 Growth: Yellow colonies
Enterococcus faecalis NCTC 12697 Growth: Pink colonies
Salmonella typhimurium NCTC 12023 Growth: Straw colonies
Klebsiella aerogenes NCTC 10006 Growth: Red-orange colonies
Pseudomonas aeruginosa NCTC 12903 Growth: Straw colonies

 

Formula*

g/L
Peptone 20.0
Lactose 10.0
Bile salts 5.0
Neutral red 0.075
Agar 13.0
Total 48.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 48g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.