Blood Agar Base No.2

SKU: KM0034 Category: Dehydrated Culture Media

Blood agar base No. 2 is a general-purpose medium enriched with various concentrations of horse or sheep blood and is suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance.
Haemolysis observations may vary with the type of blood being used. Previous studies have shown that sheep blood provides the most reliable colony and haemolysis characteristics.
The peptone, yeast extract and liver digest act as nitrogen, carbon and vitamin sources in this medium. Sodium chloride maintains osmotic balance.

Additional Information

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Beige
Prepared Medium Colour	Red
Dehydrated PH	7.4 ± 0.2
Prepared PH	7.4 ± 0.2
Recommended Incubation	Aerobically or microaerobically at 35-37°C for 18-24 hours

Product Description

Organisms	Ref. No	Result
Streptococcus pneumoniae	ATCC 6305	Growth, grey colonies with α-haemolysis
Escherichia coli	ATCC 25922	Growth, opaque / grey colonies
Streptococcus pyogenes	ATCC 19615	Growth, white colonies with β-haemolysis
Staphylococcus aureus	ATCC 25923	Growth, white colonies

Formula*

	g/L
Peptone	15.0
Yeast extract	5.0
Liver digest	2.5
Sodium chloride	5.0
Agar	12.0

Total	39.5

*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 39.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 42-46°C and aseptically add the desired concentration of horse or sheep blood and selective supplements if required. Homogenize gently and dispense into appropriate sterile containers.

Blood Agar Base No.2