Campylobacter Agar Base

SKU: KM0044 Category: Dehydrated Culture Media

This is one of several selective media available for the isolation of Campylobacter spp. in clinical, food and environmental laboratories. Campylobacter agar base is based on the formulation from Bolton and Robertson.
The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium.
The medium is enriched with lysed horse blood and made selective by the addition of cefoperazone, to suppress other enteric organisms, and amphotericin to suppress yeast and fungal growth (Preston supplement LS0010).

Additional Information

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Beige
Prepared Medium Colour	Dark Red
Dehydrated PH	7.5 ± 0.2
Prepared PH	7.5 ± 0.2
Recommended Incubation	Micro-aerophilic at 42°C for 48 hours

Product Description

Organisms	Ref. No	Result
Campylobacter jejuni	NCTC 11322	Growth, grey colonies
Campylobacter coli	ATCC 43478	Growth, grey colonies
Escherichia coli	ATCC 25922	Inhibited
Proteus mirabilis	ATCC 29906	Inhibited

Formula*

	g/L
Peptone	20.0
Sodium chloride	5.0
Agar	12.0

Total	37.0

*Adjusted/supplement as required to meet performance requirements

Preparation

Suspend 37g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 70 ml/l of lysed horse blood and 2 vials/l of Preston supplement LS0010. Mix well and dispense into appropriate sterile containers.

Campylobacter Agar Base