LB agar (Lennox) is used for the cultivation and maintenance of recombinant strains of Escherichia coli and is based on the formulation stated by Lennox. This medium is typically used in molecular biology procedures such as the detection of phage or plasmid transformed bacteria. LB agar (Lennox) is prepared using 5 g/L of sodium chloride and this level varies from that described by Miller. This allows for additional sodium chloride to be added at the point of preparation if required.
Enzymatic digest of casein provides the required nitrogen and carbon for organism growth. Vitamin B complex required by recombinant strains of E. coli are supplied by the yeast extract. Sodium chloride maintains the osmotic balance and provides sodium ions for membrane transport.
Related Supplements :
LS0035 Ampicillin Selective Supplement, LS0037 Kanamycin Selective Supplement