Letheen agar modified is intended for use in the isolation of microorganisms from cosmetics.
The casein peptone, meat peptone, beef extract and yeast extract act as carbon, nitrogen and vitamin sources in this medium.
Glucose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium.
The sodium bisulfite, lecithin and polysorbate 80 inactivates quaternary ammonium compounds.
Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.
Letheen Agar Modified
Additional Information
| Container | |
|---|---|
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
Product Description
| Organism | Ref. No. | Result |
|---|---|---|
| Escherichia coli | ATCC 8739 | Growth, cream colonies |
| Enterococcus faecalis | NCTC12697 | Growth, white colonies |
| Pseudomonas aeruginosa | ATCC 9027 | Growth, cream colonies |
| Staphylococcus aureus | ATCC 6538 | Growth, white colonies |
Formula*
| FORMULA* | g/L |
|---|---|
| Casein peptone | 10 |
| Meat peptone | 10 |
| Polysorbate 80 | 7 |
| Sodium chloride | 5 |
| Beef extract | 3 |
| Yeast extract | 2 |
| Glucose | 1 |
| Lecithin | 1 |
| Sodium bisulfite | 0.1 |
| Agar | 15 |
| Total | 54.1 |
Preparation
Suspend 54.1g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers
