Sabouraud liquid medium USP is used in sterility testing for the detection of moulds, yeasts and acidophilic microorganisms in pharmaceutical products. This medium is also used for non-sterile testing and for the determination of fungistatic activity. Sabouraud liquid medium USP conforms to the USP and Harmonised Pharmacopeia. The peptic digest of animal tissue and pancreatic digest of casein provides the required nitrogen, carbon and vitamins. The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.
Sabouraud Liquid Medium USP
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Candida albicans | ATCC 10231 | Growth |
| Aspergillus brasiliensis | NCPF 2275 | Growth. white mycelia with or without black spores |
Formula*
| g/L | |
| Peptic digest of animal tissue | 5.0 |
| Pancreatic digest of casein | 5.0 |
| Dextrose | 20.0 |
| Total | 30.0 |
| *Adjusted/supplemented as required to meet performance requirements | |
Preparation
Suspend 30g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Dispense into appropriate sterile containers and sterilise at 121°C for 15 minutes.