Mueller Hinton Agar

Mueller Hinton Agar

KM0183 Mueller Hinton Agar is used as a base to make various media for antimicrobial susceptibility testing by the disk diffusion method as described by Bauer-Kirby. First described by Mueller and Hinton, Mueller Hinton Agar has been approved as the definitive medium for antimicrobial susceptibility testing by European Committee on Antimicrobial Susceptibility Testing (EUCAST), Clinical and Laboratory Standards Institute (CLSI) and is compliant with ISO16782:2016.

KM0183, when prepared as unsupplemented Mueller Hinton Agar, is suggested by EUCAST and CLSI for non-fastidious organisms such as Enterobacterales, Pseudomonas spp., Stenotrophomonas maltophilia, Acinetobacter spp., Staphylococcus spp., Enterococcus spp., Aeromonas spp., Achromobacter xylosoxidans, Vibrio spp., Bacillus spp. and Burkholderia pseudomallei.

KM0183, when prepared supplemented with 5% horse blood and nicotinamide adenine dinucleotide (NAD), is suggested by EUCAST and CLSI for fastidious organisms such as Streptococcus pneumoniae, Streptococcus groups A, B, C and G, Viridans group streptococci, Haemophilus influenzae, Moraxella catarrhalis, Listeria monocytogenes, Pasteurella multocida, Campylobacter jejuni and coli, Corynebacterium spp., Aerococcus sanguinicola and urinae and Kingella kingae.

Additionally, according to the UK Standards for Microbiology Investigations, when supplemented appropriately, Mueller Hinton Agar can be used in the process of identifying Haemophilus species and the HACEK group of organisms and Helicobacter species from clinical specimens.

Related Supplements :
LS0005 NAD Supplement (20mgs/L), Defibrinated Horse Blood

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

No Supplementation

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth: White colonies
Escherichia coli NCTC 12241 Growth: Opaque/grey colonies

 

With 5% horse blood & NAD

Organisms Ref. No Result
Haemophilus influenzae NCTC 12975 Growth: Opaque/grey colonies
Streptococcus pneumoniae NCTC 12977 Growth: Grey/green colonies with α-haemolysis

Formula*

g/L
Beef extract 2.0
Acid hydrolysate of casein 17.5
Starch 1.5
Agar 17.0
Total 38.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 38.0g of dehydrated culture medium in 1 litre of deionised/purified water and allow to soak whilst mixing for 10 minutes. Autoclave to sterilise at 121°C for 15 minutes before cooling in a water bath to 45-50°C. If preparing a blood Mueller Hinton plate, after cooling aseptically add the appropriate volumes of defibrinated horse blood (E&O DHB) and NAD supplement (E&O LS0005). Gently homogenize the final medium before aseptically dispensing the specified volume into appropriate sterile containers and allowing to cool.