Sheep Blood Agar Base

SKU: KM0189 Category: Dehydrated Culture Media

Sheep Blood Agar Base has been developed to provide a nutrient rich medium compatible with sheep blood for the cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions.
Alternative culture mediums, such as Blood agar base No. 2, can results in mixed haemolytic reactions for some Streptococcus spp. This is thought to occur due to the trace amounts of fermentable carbohydrates in yeast extract and the physiological difference between sheep and horse blood.
The tryptone, peptone, and yeast extract provide the required nitrogen, carbon and vitamins in the medium. Sodium chloride maintains the osmotic balance.

Related Supplements :
LS0008 Staph/Strep Selective Supplement

Additional Information

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Straw
Prepared Medium Colour	Red
Dehydrated PH	7.3 ± 0.2
Prepared PH	7.3 ± 0.2
Recommended Incubation	Aerobically at 30-35°C for 18-24 hours

Product Description

Organisms	Ref. No	Result
Streptococcus pyogenes	NCTC 12696	Growth, white colonies with β-haemolysis
Streptococcus pneumoniae	ATCC 6305	Growth, grey/green colonies with α-haemolysis
Staphylococcus aureus	NCTC 12981	Growth, white colonies
Escherichia coli	NCTC 12241	Growth, opaque/grey colonies

Formula*

	g/L
Tryptone	14.0
Peptone	4.5
Yeast extract	4.5
Sodium chloride	5.0
Agar	12.0
Total	40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. Once cooled add 5 – 10% of defibrinated sheep blood and mix well. Aseptically dispense into appropriate sterile containers.

Sheep Blood Agar Base