Escherichia coli 0157 Selective Supplement
E&O Laboratories Ltd Escherichia coli 0157 Selective Supplement (LS0013) is an antibiotic supplement used to enhance the isolation of Escherichia coli serogroup O157 from faecal, food and environmental specimens.
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Solubility Description | |
| Recommended Incubation |
| Organisms | Ref. No | Result |
| Escherichia coli 0157 | NCTC 12900 | Growth |
| Escherichia coli | ATCC 25922 | Inhibited |
Each 500ml vial contains:
| Cefixime | 0.05mg |
| Potassium Tellurite | 2.5mg |
To reconstitute the contents of each vial, aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and add the contents of the vial to 1 Lt of sterile Sorbitol MacConkey Agar Base prepared according to the manufacturer’s instructions and allowed to cool to 45°- 50°C. Mix gently but thoroughly and pour aseptically into sterile Petri Dishes.
Overnight storage at 2°- 8°C is recommended to allow equilibration of the supplement within the medium.