Cefotaxime Selective Supplement
E&O Laboratories Ltd Cefotaxime Selective Supplement can be used with MacConkey Agar for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing organisms with the aim of simplifying the differentiation and presumptive identification of Lactose & Non-Lactose fermenting organisms. It should be noted that AmpC isolates may also be detected on this medium whilst non – ESBL organisms will be inhibited on this medium.
Sodium Chloride is omitted from this medium to provide an electrolyte deficient medium that prevents Proteus spp. from spreading.
Cefotaxime Selective Supplement (1mg/L)
Additional Information
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Product Description
| Organisms | Ref. No | Result |
| Test as PP0477 | ||
| Escherichia coli | ESBL 1 | Growth |
| Escherichia coli | NCTC 12241 | Inhibited |
| Proteus mirabilis | NCTC 10975 | Inhibited |
| Staphylococcus aureus | NCTC 12981 | Inhibited |
Formula
Each vial contains Cefotaxime at a concentration of 0.001gms/litre
Procedure
To reconstitute each vial aseptically add approximately 2ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution.
Add the contents of the vial to the required sterile base medium of choice prepared according to the manufactures instructions and allowed to cool to below 50°C. Mix gently but thoroughly and pour or aseptically dispense into the desired sterile containers or dishes.