Primary Listeria Selective Supplement
E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Solubility Description | |
| Recommended Incubation | Incubate plates at 35-37°C in aerobic atmosphere for 36-48 hours |
| Organisms | Ref. No | Result |
| Listeria monocytogenes | NCTC 11994 | Growth |
| Listeria innocua | NCTC 11288 | Growth |
| Enterococcus faecalis | NCTC 12697 | Inhibited |
| Saccharomyces cerevisiae | NCPF 3178 | Inhibited |
| Escherichia coli | NCTC 12241 | Inhibited |
| Vial Contents | Per Vial | Per Litre |
| Ceftazidime | 10.0mg | 20mg |
| Amphotericin B | 5.0mg | 10mg |
| Polymixin B | 5.0mg | 10mg |
| Nalidixic acid | 10.0mg | 20mg |
To reconstitute the contents of each vial, aseptically add 2ml of Sterile Deionised Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be a, yellow colour and free from particulate matter. Immediately add the contents of the vial to 500ml of sterile Primary Listeria Selective agar prepared according to the manufacturer’s instructions and allowed to cool to 45- 50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes.