Modified Campylobacter (Bolton) Selective Supplement
E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
Modified Campylobacter (Bolton) Selective Supplement
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Solubility Description | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
Formula
Each 500ml vial contains the following raw materials:
| Vancomycin | 10mgs |
| Trimethoprim | 10mgs |
| Cefoperazone | 10mgs |
| Amphotericin B | 5mgs |
Procedure
To reconstitute the contents of each vial, aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and add the contents of the vial to an appropriate volume of sterile base medium of choice (Columbia Agar with 5-7% Lysed Horse Blood is recommended) prepared according to the manufacturer’s instructions and allowed to cool to 44-50°C. Mix gently but thoroughly and pour aseptically into sterile Petri dishes. Overnight storage at 2-8°C is recommended to allow equilibration of the supplement within the medium.
For extended storage at 2-8°C plates should be contained in sealed plastic sleeves or similar packing.
NB: Campylobacter Growth Supplement (LS0011) may also be used when preparing this medium.