Legionella BCYE with GVPC

This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram negative organisms and Cycloheximide is also included to inhibit yeasts and fungi.