Brazier’s CCEY Agar with 1% Horse Blood Lysed, Cycloserine, Cefoxitin & 4% Egg Yolk Emulsion

Brazier’s CCEY Agar with 1% Lysed Horse Blood – Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion
Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light.