Primary Listeria Diagnostic Supplement

BM8050 Primary Listeria Diagnostic Supplement is used to enhance the differentiation of Listeria monocytogenes from other Listeria spp. found in food and environmental samples. BM8050 is designed to be used with E&O KM0058 to form Primary Listeria Agar.

Listeria monocytogenes is commonly found in soil, sewage, and faeces. It is difficult to eradicate, and can cause serious food poisoning; therefore, L. monocytogenes is frequently tested for in food processing facilities to avoid contamination. Contamination can occur at all steps of the food manufacturing chain from raw materials to point of consumption.

Primary Listeria Agar is used for the detection and presumptive identification of Listeria spp. and the specific differentiation of L. monocytogenes. Based on the method of Ottaviani et al ^{(1 2)}, this medium allows detection and enumeration of Listeria spp. as early as 24 hours. Primary Listeria Agar is recommended by ISO 11290-1:2017 ⁽³⁾ and ISO 11290-2:2017 ⁽⁴⁾ and tested in accordance with ISO 11133:2014 ⁽⁵⁾.

1. Ottaviani, F., Ottaviani, M., and Agosti M. (1997) Differential agar medium for Listeria monocytogenes. Quimper Froid Symposium Proceedings, P6 ADRIA Quimper, France, 16-18 June.
2. Ottaviani, F., Ottaviani, M.G., and Agosti, M. (1997). Esperienze su un agar selettivo e differenziale per Listeria monocytogenes. Industrie Alimentari, 36: 888-889.
3. International Organization for Standardization (2017) 11290-1:2017 Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 1: Detection method. Geneva, ISO.
4. International Organization for Standardization (2017) 11290-2:2017 Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 2: Enumeration method. Geneva, ISO.
5. International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media. Geneva, ISO.