Traditionally, membrane Lauryl Suphate Broth (mLSB) was used as the standard media for isolating coliforms (including E. coli) from drinking water. Primary membrane Lactose Glucuronide Agar (mLGA) is a chromogenic modification of mLSB formulation aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and <em,>E. coli . The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. X-glucuronide is incorporated to allow for the presumptive isolation of E. coli, sodium pyruvate aids recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads.
This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Environment Agency’s – The Microbiology of Drinking Water 2009 (Part 4).
Primary mLGA
Additional Information
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| Recommended Incubation | Incubate aerobically at 30 ± 1°C for 4 hours followed by a minimum of 14 hours at 37 ±1°C. |
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Product Description
| Organisms | Ref. No | Result |
| Escherichia coli | NCTC 12241 | Green colonies |
| Citrobacter freundii | ATCC 43864 | Yellow colonies |
| Salmonella typhimurium | NCTC 12023 | Red colonies |
| Enterococcus faecalis | NCTC 12697 | Inhibited |
Recommended incubation: Incubate aerobically at 30 ± 1°C for 4 hours followed by a minimum of 14 hours at 37 ±1°C.
