This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction.
Thermostable Nuclease Test (TSN) Agar
Additional Information
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| Recommended Incubation | Recommended incubation: Incubate aerobically at 37 ± 1°C for 4 hours. |
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Product Description
| Organisms | Ref. No | Result |
| Staphylococcus aureus | NCTC 12981 | Zone of clearing around inoculation well |
| Staphylococcus epidermidis | NCTC 13360 | No zone of clearing around inoculation well |
