Tryptone yeast extract salts (TYES) agar is a culture medium that can be used as the primary isolation medium for Flavobacterium columnare (1) and F. psychrophilum.
Growth of Flavobacterium spp. requires a culture media with a lower nutrient content than that used in general-purpose mediums such as brain heart infusion agar andtryptone soya agar.
The tryptone and yeast extract provide the required carbon, nitrogen and vitamins.
Calcium chloride and magnesium sulphate provide the required minerals.
Agar is a solidifying agent.
Tryptone Yeast Extract Salts (TYES) Agar
Additional Information
| Container | |
|---|---|
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Flavobacterium columnare | DSMZ 18644 | Growth, yellow colonies |
| Flavobacterium psychrophilum | DSMZ 3660 | Growth, yellow colonies |
Recommended incubation: Aerobically at 15-25°C for 1-5 days
Formula*
FORMULA* g/L
Tryptone 4.0
Yeast extract 0.5
Calcium chloride 0.2
Magnesium sulphate 0.5
Agar 10.0
Total 15.2
* Adjusted / supplemented as required to meet performance requirements
Preparation
Suspend 15.2g of the medium in one litre of deionised / purified water.
Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 minutes to completely dissolve the medium.
Cool to 45-50°C, mix well and dispense into appropriate sterile containers
